The Quantitative Measurement of DNA Hybridization from Renaturation Rates
نویسندگان
چکیده
منابع مشابه
Studies on the spectrophotometric determination of DNA hybridization from renaturation rates.
The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreeme...
متن کاملCharacterization of denaturation and renaturation of DNA for DNA hybridization
OBJECTIVES The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. METHODS A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was de...
متن کاملHybridization and renaturation kinetics of nucleic acids.
When two DNA or RNA molecules contain sequences of nucleotides that are complementary to one another, they are able to come together to form a double helix with all of the bases paired. If both strands are DNA, the process is usually called renaturation or reassociation; if one strand is DNA and the other is RNA, the process is called hybridization. These essentially identical processes form th...
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15 صفحه اولKinetics of renaturation of DNA.
The rate of renaturation of fully denatured DNA is kinetically a second-order reaction. The reaction rate increases as the temperature decreases below T,t, reaching a broad flat maximum from 15 to 30°C below T, and then decreases with a further decrease in temperature. Let N be the complexity of the DNA or the number of base-pairs iu non-repeating sequences per virus or cell for the given DNA, ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1970
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1970.tb00830.x